Nucleoproteins. 2. Fractionation of deoxyribonucleoproteins into acidic proteins and lysine-rich histones associated with deoxyribonucleic acid
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چکیده
منابع مشابه
Amino acid sequence and sequence variability of the amino-terminal regions of lysine-rich histones.
The amino acid sequence has been determined for the first 72 residues of a lysine-rich histone from rabbit thymus. These are the residues contained in a fragment released from the histone by treatment with N-bromosuccinimide. Peptides derived by tryptic, thermolysin, and chymotryptic digestion of this 72-residue fragment were used to reconstruct the total sequence. Analysis of the sequence reve...
متن کاملSpecies and organ specificity in very lysine-rich histones.
Very lysine-rich histones were extracted by aqueous trichloracetic acid from calf thymus, from the thymus, mammary gland, and liver of rabbits, and from chicken livers. The elution profiles of these histones, obtained by chromatography on Amberlite IRC-50 with a shallow guanidinium chloride gradient, revealed that the very lysine-rich histone complement varied from one animal species to another...
متن کاملSpecies and Organ Specificity in Very Lysine-rich Histones*
Very lysine-rich histones were extracted by aqueous trichloracetic acid from calf thymus, from the thymus, mammary gland, and liver of rabbits, and from chicken livers. The elution profiles of these histones, obtained by chromatography on Amberlite IRC-50 with a shallow guanidinium chloride gradient, revealed that the very lysine-rich histone complement varied from one animal species to another...
متن کاملImmunological specificities of lysine-rich histones from tumors.
The specificity of Fr histones from tumors was investigated with the following immunological probes: antisera elicited by each of the Fr subfractions obtained from rat thymus; antisera elicited by purified unfractionated rat hepatoma FI histones; and antisera elicited by the COOH-terminal (N1) fragment of rat hepatoma F1 histones. The results indicate that Fr histones derived from tumors retain...
متن کاملDeoxyribonucleoproteins and the tissue-specific restriction of the deoxyribonucleic acid in chromatin.
Although histones appear necessary for the restriction of DNA (Bonner et al. 1968), their lack of tissue and species specificity point to the necessary presence of other factors in chromatin that are required for the highly organ-specific genetic restriction of the DNA. The present paper analyses the type of restriction of the DNA in native chromatin by analysing the type of restriction in hybr...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1964
ISSN: 0006-2936
DOI: 10.1042/bj0900578